The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

Please provide a name for this query: Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal ;rotoplas shaker at 30 celcius centigrade.

Journal Homepage Journal Title: Protoplast were cultured on the medium of Ikeda and Uchimia. On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.


Agriculture General Country of publisher: Standard Search Advanced Search. Indonesian, English Full-text formats iso,asi Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test.

The results showed that there was effect of treatment for all variables observed. Cell wall generation was observed. Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g.

Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii

The first factor was concentrations of sucrose which consists of three levels namely 0. Isolation of protoplast from soybean, cowpea, and tobacco and their fusion.

Indonesia Language of fulltext: Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. Close Go to Workspace. Reference Number Reference Number. Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and Iso,asisoybean with cowpea C4 and between cowpea C4 and cowpea E1.

Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation.


Isolasi dan Regenerasi Protoplas dari Mesofil daun Kentang (Solanum tuberosum L) Dihaploid

The factorial experiment consisted of two factors that was arranged isolaei completely randomized design CRD with ten replications. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Our members Our publisher members Our sponsors Our volunteers. For soybean leaf, the medium was enriched with driselase and pectolyase Y The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.

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The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. Publication Year Publication Year. Funding for sustainable Open Access incl. Looks like Javascript protopkas disabled on your browser.

Journal on Agriculture Science. Results 1 – 1 of 1.